Coding

Part:BBa_K1975006

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-10-05)


Red Fluorescent Protein

This is Red Fluorescent Protein that can be used to tag proteins of interest. It is a fluorescent protein that will show a red color when it is excited at 558nm and it has the highest emission at 583nm. This sequence has been used to tag PhaB to visualize the expression of the protein. If the protein was expressed correctly, the cells would show a red color when excited. The sequence is optimized for use in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 433
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None